Sr. Research Associate - Library Cloning; AAV Engineering

The Company

Dyno Therapeutics is reshaping the gene therapy landscape through AI-powered vectors. Through the application of our transformative technologies and strategic partnerships with leaders in gene therapy, we believe a future with life changing gene therapies for millions of people is within reach.

Our team includes world-class molecular and synthetic biologists, protein engineers and gene therapy scientists working alongside software engineers, data scientists, and machine learning experts.Dyno was named Startup of the Year in 2020 by Xconomy!

The Role

Sr. Research Associate - Library Cloning; AAV Engineering. Under the guidance of senior researchers, you will support a number of stages in the Dyno pipeline, including the assembly of highly complex AAV vector libraries from synthesized DNA and the preparation of AAV library samples for Next-Gen Sequencing analysis. Consistency, clever protocol optimization, and precision are key to Dyno's workflow.

How You Will Contribute

As a member of the scientific team,  you will work with our vector engineering process on both ends. This position calls for an individual with established cloning and Molecular Biology skills, and the ability to learn, develop, and optimize detailed protocols as the need arises. You will collaborate with scientists and computational biologists throughout Dyno - learning from and contributing to a diverse set of projects. You will be responsible for understanding project goals and for creatively developing solutions to ensure their success.

Responsibilities: 

  • Clone highly complex library assemblies for AAV production
  • Execute large scale DNA preparation protocols for transfection
  • Implement next-gen sequencing DNA preparation procedures (amplification/ligation)
  • Presentation of results in platform team and company-wide meetings
  • Assist in lab organization, including ordering supplies and restocking 
  • Follow lab protocols and safety rules

Who you are

  • Trusted partner
  • Team oriented
  • Thoughtful & detail oriented 
  • Work with a sense of urgency
  • Appreciation for opportunities at the intersections of data science and biology

Basic qualifications

  • BA or MA in biology, biochemistry, biomedical engineering, or related fields
  • Minimum 2+ years full-time wet lab experience, 3+ years full-time professional laboratory experience preferred 
  • Highly skilled in the following Molecular biology skills:
    • Molecular cloning (ligation, Golden Gate / Gibson assembly, electroporation, plating, DNA purification, Sanger sequencing)
    • PCR
    • Agarose gel electrophoresis and imaging
  • Knowledge of oligo/primer/probe/gBlock design strategies
  • Ability to conduct advanced protocols and procedures precisely and consistently
  • Ability to document and maintain an organized account of lab work and results

Preferred qualifications

  • Focused experience in advanced DNA cloning methodologies, especially with complex DNA libraries (e.g. protein variant libraries, sgRNA libraries, etc.)
  • Focused experience in NGS DNA library generation to characterize library composition 
  • Focused experience in RNA/DNA quantification (qPCR) and quality control
  • Cell culture skills: maintenance, transfection, virus titration, virus transduction
  • Sequence design and analysis software skills, such as Benchling and BLAST 

We are an equal opportunity employer and value diversity at our company. We do not discriminate on the basis of race, religion, color, national origin, gender, sexual orientation, age, marital status, veteran status, or disability status.

Job Type: Full-time

Posted
10/02/2021
Location
Cambridge, MA 02140, US
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